Abstract

STUDIES ON CYTOTOXIC EFFECT OF ALBIZIA LEBBECK METHANOLIC BARK EXTRACT ON HUMAN CELL LINES

Ariharasivakumar Ganesan* and Kavimani Subramanian

Abstract

To investigate cytotoxic effects and the mechanism of cell death of Albizia lebbeck bark extract HeLa, Miapaca-2, HepG2, AGS, U373MG, A431, NIH3T3 and MCF7 cell lines was examined. MTT (3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were performed in cell line as well as human endothelial cells to analyse the cytotoxic activity of the extracts of Albizia lebbeck. The apoptosis inducing action of the extracts was determined by TUNEL test and cell death assay. The results showed that the methanol extract significantly inhibited cell growth and viability in dose dependent manner without inducing damage of non- cancerous cell lines indicated generation of methanol extract of Albizia lebbeck in MCF-7cells. This study may be containing of bark extract isolated in bioactive compounds for the treatment of cancer.

Keywords: Albizia lebbeck bark, Human cell line.