Abstract

ALPHA LIPOIC ACID POTENTIATE THE ANTIEPILEPTIC ACTIVITY OF PHENOBARBITAL IN EPILEPTIC MICE.

R. S. Cheke, P. R. Todkari, N. P. Sawadadkar*, V. J. Chaware, K. R. Biyani

Abstract

Phenobarbital is most commonly used antiepileptic drug. But most of patient suffers from the side effects of Phenobarbital. For the safety and efficacy of this drug it is necessary to identify the adjuvant therapy that could increase the therapeutic effect. One potential therapy is Alpha Lipoic Acid. Alpha Lipoic Acid is a biologic antioxidant which also used to decrease the oxidative stress produced in epilepsy. The main aim of present study to potentiate the antiepileptic activity of Phenobarbital and also reduced the dose of Phenobarbital in such a way that that could not affects its therapeutic benefit with the alpha lipoic acid which is used as adjuvant. The reduced dose of Phenobarbital could reduces the side effects. In these study all the animals were divided into sixteen groups & each groups consist of six animals. All group received PTZ (65mg/kg i.p.). Mice were pre treated with Phenobarbital and alpha lipoic acid. Testing drugs doses were randomised within groups of animal such that each group was required during a given test series. The pharmacological screening shows that Phenobarbital, co-administration of Phenobarbital & alpha lipoic acid significantly increased latency to clonic convulsions & reduced mortality in PTZ treated. Also alpha lipoic acid, Phenobarbital combination treatment decreased serum MDA activity. It is concluded that alpha lipoic acid potentiates the anti epileptic activity of Phenobarbital in mice. Alpha lipoic acid, Phenobarbital in combination decreased oxidative stress and prevents epilepsy also the reduced dose of Phenobarbital causes reduction in side effects.

Keywords: ALA, PTZ, PB, Seizures, MDA.