Abstract

HERBAL CASTRATION BY MEANS OF ORAL ADMINISTRATION OF GREEN TEA LEAF EXTRACT (Camellia sinensis L.) ON MALE REPRODUCTIVE SYSTEM

Shyamal Kanti Das* and Soumendra Nath Karmakar

Abstract

Aqueous extract of green tea leaf, used in this experiment has shown its impact on male reproductive system. Various blood parameters have been changed and the structure of testis is altered after oral administration of Green Tea Leaf Extract (GTLE). The extract was prepared according to the method of Wei.H.et al (1999). The extract was given to two different experimental animal groups with two different doses during 26 consecutive days. After oral administration of GTLE, it was found that, the weight of the testis and epididymis as accessory sex organ were markedly reduced instead of normal weight gain of all the animals. The sperm count and motility were reduced for the treated groups as compared with control animal group. The enzymes like SGPT and SGOT were as usual and other blood parameters like glucose and protein were also as usual comparing with controlled group. Cholesterol level in the serum was insignificantly altered in dose treated groups. Testosterone level was reduced in the treated groups. FSH and LH levels were also altered accordingly in treated groups. Histological examination showed inhibition of spermatogenesis as evidenced by disintegration of seminiferous tubules of testis. Result of this study showed that GTLE has potent castrative activity on male reproductive system in dose dependent manner.

Keywords: Green tea leaf extract, castrative agent, epigallocatechin, sperm motility, steroidogenic enzyme.