Abstract

QUANTITATIVE DETERMINATION OF SWERTIAMARIN, MANGIFERIN AND AMAROGENTIN IN CALLUS CULTURE OF SWERTIA CHIRATA BY HPLC ANALYSIS

Anil Kumar* and Shivam Sharma

Abstract

The paper deals with the quantitative determination of swertiamarin, mangiferin and amarogentin contents in Swertia chirata by HPLC analysis. The extracted plant material (20mg) was transferred in to 15ml borosil test tubes. 20ml of methanol was added and the tubes were capped securely. The content was heated in a water bath at 80°c in order to extract metabolites of interest. The extract was filtered and the residue was re-extracted twice in 20ml of methanol. The final volume was made up to 10ml by evaporation. The methanol extracts were combined and filtered through 0.22μ filter membrane. The solvents, namely water and acetonitrile used in the study were filtered and degassed. This was essential since storage of the solvent cause gas bubbles to accumulate. The results showed that the callus culture contained 0.67% swertiamarin, 0.09% mangiferin and 0.05% amarogentin and in contrast, tissue cultured plants contained 0.71% swertiamarin, 1.04% mangiferin and 0.08% amarogentin. On the other hand 2.91% swertiamarin, 0.07% mangiferin and 0.08% amarogentin was found in field grown plants of Swertia chirata.

Keywords: Swertia chirata, swertiamarin, HPLC, mangiferin, amarogentin.