Abstract

COMPARATIVE ANALYSIS AND SIMULTANEOUS QUANTIFICATION OF ANTIOXIDANT CAPACITY OF FOUR TERMINALIA SPECIES USING VARIOUS PHOTOMETRIC ASSAYS

Kalpna Rakholiya, Mital Kaneria, Krunal Nagani, Ankita Patel and Sumitra Chanda*

Abstract

Most of the Terminalia species are a popular folk medicine and has several proven biological properties. Present study was performed for comparative analysis and simultaneous quantification of antioxidant properties of leaf and stem of four Terminalia species belongs to the family Combretaceae. Plant material was extracted by individual cold percolation extraction method using various solvents. The antioxidant activity of all the different solvent extracts of leaves and stem was evaluated using various photometric antioxidant assays like DPPH free radical scavenging assay, superoxide anion radical scavenging assay, ABTS radical cation scavenging assay and Ferric reducing antioxidant power (FRAP). Total phenol and flavonoid content was also measured. The results showed that the extracting solvent significantly altered the antioxidant property estimations of leaf and stem. High correlations between phenol content and antioxidant activities of extracts were observed. High levels of antioxidant activities were detected in T. chebula as compared to the other species indicating that T. chebula may serve as an excellent source of natural antioxidants.

Keywords: Terminalia, leaf, stem, antioxidant, individual extraction, TPC, FC, FRAP, DPPH, ABTS, superoxide