Abstract

HISTOPATHOLOGICAL CHANGES OF AUGMENTATION CYSTOPLASTY WITH ACELLULAR HUMAN AMNIOTIC MEMBRANEIN RABBITS

Seyed Mokhtar Hosseini, Dinyar Khazaeli, Hadi Naddaf, Babak Mohammadian, Saeid Hosseini*, Ali Goudarzikarim

Abstract

We aimed toevaluate the histopathological changes in HumanAmniotic membrane in respect of transitional metaplasia or infiltration, and smooth muscle creation underthe transitional layer,following Augmentation cystoplasty with human amniotic membranein rabbits. Materials and Methods:This study was conducted on 12 healthy New Zealand white female rabbits aged between 12-15 months with the weight of approximately 3- 3.5 Kg. Freshamniotic membrane grafted at the dorsal wall of rabbits bladder. Then the histopathological changes of the graft assessed every two weeks over threemonths. Results: After Augmentation cystoplasty with amniotic membrane, thereconstruction of transitional epithelium layer of bladder, lamina propria, muscle layer and neovascularization, observed through granulation tissue proliferation and repithelialization withmild to severe inflammatory response, woundcontraction and necrosis. Conclusion:Amniotic membrane was necrosed without metaplasia and transitional layer was repaired with repithelialization, also the smooth muscle was created under transitional layer. Amniotic membrane as a biologic scaffold can form a base membrane for bladder reconstruction.

Keywords: Augmentation cystoplasty, amniotic membrane, repithelialization, scaffold, transitional layer.