Abstract

EVALUATION OF POLYHERBAL PREPARATION OF LIVINA AGAINST ETHANOL INDUCED LIVER DYSFUNCTION

Manoj Kumar Sawner*, Dr. Ankita Shukla

Abstract

Background: Ethanol-induced hepatic dysfunction is associated with oxidative stress, membrane damage, and metabolic imbalance. Polyherbal formulations may offer hepatoprotective benefits through multi-component antioxidant and cytoprotective mechanisms. Objective: To assess the protective potential of the polyherbal formulation Livina against ethanol-induced hepatocellular injury using in-vitro and laboratory-based methods. Methods: Livina was evaluated for quality/standardization parameters (organoleptic evaluation, physicochemical tests, phytochemical screening, and chromatographic fingerprinting). Antioxidant potential was assessed using assays such as DPPH/ABTS/FRAP and total phenolic/flavonoid content. Hepatoprotective activity was assessed in a hepatocyte cell model (e.g., HepG2/primary hepatocytes) exposed to ethanol, using cell viability (MTT/resazurin), membrane integrity (LDH leakage), intracellular ROS (DCFH-DA), and antioxidant markers (GSH, SOD, CAT) in cell lysates. Results: Livina showed measurable antioxidant capacity and reduced ethanol-induced cytotoxicity by improving cell viability, reducing LDH leakage, lowering ROS generation, and restoring antioxidant defenses (p < 0.05). Conclusion: Livina exhibited significant in-vitro hepatoprotective potential against ethanol-induced cellular injury, likely mediated through antioxidant and membranestabilizing effects.

Keywords: Livina; polyherbal; ethanol; hepatoprotection; oxidative stress; HepG2; ROS; LDH.