Abstract

AN INTEGRATED ANALYTICAL STRATEGY FOR SELECTED ANTIBACTERIAL COMPOUNDS USING UV-VISIBLE SPECTROSCOPY: A REVIEW

*V. Nandhini Priya, Dr. R. Manivannan, P. Deepak, B. Kajal, K. Kanishka,

Abstract

Background: UV-Visible spectroscopy is a widely employed analytical technique in pharmaceutical analysis for the identification and quantification of antibacterial compounds. Its simplicity, cost- effectiveness, and precision make it a standard method in quality control and research settings. bjective: To review the principles, instrumentation, and integrated analytical application of UV-Visible spectroscopy for the analysis of selected antibacterial compounds including Ciprofloxacin, Amoxicillin, Doxycycline, and Metronidazole. Methods: This review integrates findings from UV-Visible spectroscopic principles including Beer- Lambert Law, electronic transitions, instrumentation components, and spectral data obtained for the elected antibacterial drugs. Structural elucidation using ChemDraw software and calibration curve methodology are also incorporated. Results: UV-Visible spectroscopy demonstrated consistent linearity for all four antibacterial compounds analyzed. Amoxicillin showed a UV absorbance peak at 272 nm with a linear range from 5 to 25 μg/mL (absorbance values 0.226 to 1.126), confirming compliance with Beer-Lambert Law. Structural characterization of each drug was successfully performed using ChemDraw software, and synthesis pathways were established and validated. Conclusion: UV-Visible spectroscopy represents an efficient, reliable, and integrated analytical strategy for the characterization and quantitative determination of selected antibacterial compounds. The method offers a favorable balance between simplicity and accuracy, supporting its continued application in pharmaceutical quality control.

Keywords: UV-Visible spectroscopy; antibacterial compounds; Beer-Lambert Law; Ciprofloxacin; Amoxicillin; Doxycycline; Metronidazole; pharmaceutical analysis; calibration curve; ChemDraw.