Abstract

BIXA OREALLANA. L: A STUDY OF PHYTOCHEMICAL, CYTOTOXIC AND ANTIOXIDANT ACTIVITY PROFILE OF THE LEAF EXTRACTS OF THE PLANT.

Sunita Bhatnagar*, Sasmita Swain and Manasi Sahoo

Abstract

Solvent extracts of Bixa oreallana leaves were tested for phytochemical, antioxidant, and cytotoxic activity. In phytochemical analysis aqueous extract showed the presence of tannins, saponins, flavanoids, terpenoids, steroids and hydroquinone. Brine shrimp assay was done to study the cytotoxic activity followed by MTT reduction assay, a mild cytotoxic activity was observed in ethyl acetate extract. Antioxidant activity was conducted using TLC based DPPH assay, Chloroform and ether extracts showed significant antioxidant activity followed by acetone and methanol extracts with similar antioxidant profile. Extracts lacked cytotoxic potential as depicted by brine shrimp assay.

Keywords: cytotoxic, antioxidant, extracts, phytochemical.