Abstract

COLLAGENASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL PROFILE OF LEAF OF JUSTICIA GENDARUSSA

S. S. Patel* and M. N. Zaveri

Abstract

Ethnomedicinally Justicia gendarussa is used for treatment of chronic rheumatism. The alcoholic extract of leaf was reported for its antiinflammatory and antiarthritic activity. This research is aim to find collagenase inhibitory fraction. The leaf was fractionated based on polarity. Methanolic extract and its fractions of leaf of J. gendarussa were screened phytochemically. Based on phytochemical screening the resultant fractions were evaluated for their collagenase (Matrix metalloproteinases) inhibitory activity at 100, 250, 500 and 1000 μg /ml concentration. Among the tested extracts, toluene fraction of leaf of J. gendarussa showed maximum Matrix metalloproteinases inhibition with IC50 value 44.75μg /ml. Further, the phytochemical analysis of isolated compounds indicated the alkaloids and saponins in leaf of J. gendarussa respectively. The Matrix metalloproteinases inhibitory activity may be because of high content of alkaloids and saponins. Based on Matrix metalloproteinases inhibitory activity, it could be suggested that the toluene fraction of J. gendarussa might have an influence on arthritis.

Keywords: Matrix metalloproteinases, Collagenase, Toluene fraction, Leaf.