Abstract

ANTIOXIDANT POTENTIALS OF FRESH JUICE OF CYNODON DACTYLON L PERS (DURVA)

Dr. Sharda Singh, *Dr. Shashi Kant Pandey, Dr. Kamal Nayan Dwivedi, Dr. Yamini
Bhushan Tripathi and Amit Kumar Sharma

Abstract

Aim of the study was to analyze the free radical scavenging activity of fresh juice of Cynodon dactylon. Juice was extracted from fresh plant of Cynodon dactylon, which was then allowed to freeze drying at reduced pressure, in a lyophilizer, to prevent degradation of constituents of juice. Powder form of juice thus obtained was used to perform various tests for invitro free radical scavenging activity that was determined by superoxide radical scavenging, lipid peroxidation assay, nitric oxide radical scavenging using UV spectrophotometer. EC50 values for superoxide radical scavenging, lipid peroxidation assay, nitric oxide radical scavenging was 197.34 ± 4.36, 685.53 ± 7.04, 298.01±4.12 respectively with reference to standards having EC50 41.70±2.40, 27.2±1.02, 63.11±3.67. The results show that fresh juice of Cynodon dactylon is a potential source of natural antioxidant.

Keywords: free radical scavenging, fresh juice, spectrophotometer, antioxidant.