Abstract

DETERMINATION OF PHYTOCONSTITUENTS OF BLEPHARIS REPENS (VAHL) ROTH LEAVES AND FRUIT THROUGH GC-MS

*Dhawale P. G. and Ghyare B. P.

Abstract

Phytochemical constituents are responsible for medicinal activity of plant species. Hence in the present study phytochemical constituents presents in the Blepharis repens leaves and fruit was identified by using Gas Chromatography – Mass Spectroscopy (GC-MS) technique. The leaves and fruits were collected, dried in shade and converted to the powder form. This powder is then extracted through Soxhlet apparatus using acetone solvent for GC-MS analysis. The concentrated extract is then analysed by GC-MS technique and the various secondary metabolites were identified using NIST library search. The result shows the presence of different phytoconstituents which includes The leaves and fruit of Blepharis repens observed for the presence of important secondary metabolites like fatty alcohol, unsaturated fatty acid, diterpene, triterpene, flavonoid, steroid and vitamin-E. These act as antimicrobial, antioxidant, anticancer, diuretic, antiasthma, antiarthetic, anti-inflamatory, antifertility, antistroke, chemo-preservative, immunostimulent and perfumery. Blepharis repens leaves and fruits contain the various phytoconstituent that can be useful in different fields like pharmaceuticals, perfume, drug development etc. and plant can be recommended as a plant of phytopharmaceutical importance.

Keywords: Blepharis repens, phytomedicines, phytoconstituents, GC-MS, secondary metabolites.