Abstract

PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTICANCER, ANTIOXIDANT ACTIVITY OF CRUDE LEAF EXTRACT OF VITEX NEGUNDO LINNIN EXPERIMENTAL ANIMAL

*Kayande Nandu and Dr. Patel Rakesh,

Abstract

The anticancer and antioxidant activity crude leaf extract of vitex negundo linn evaluated against Dalton’s ascetic lymphoma cell line and antioxidant activity for measurement of free radical scavenging activity (RSA) [reactions with 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and assessment of the influence of extracts on the enzyme xanthine oxidase (XO), and Fe3+ reducing ability. Anticancer activity was performed on swiss albino mice at the dose of 250, 500 and 1000 mg/kg. body weight. The experimental parameters used were tumour volume, tumour cell count, viable tumour cell count, mean survival time and increase in life span to assess antitumour activity. The extract administered orally for 14 consecutive days to tumor bearing group of animals. The extract increase the life span of DAL treated mice and restore the hematological parameters as compared with the DAL bearing mice in dose dependant manner. The study revealed that the EVN showed significant antitumour activity in tested animal models. Antioxidant assessment shows significant RSA and XO and extract were able to reduce fe3+.

Keywords: Anticancer, Antioxidant, RSA, Xanthine oxidase, DPPH, Vitex Negundo linn.