Abstract

IN VITRO ANTIFUNGAL, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL FROM THE AERIAL PARTS OF AGRIMONIA AITCHISONII SCHONBECK TEMESY FROM HIMALAYAN REGION

Mohammad S. Javed*, Pawan Kumar, Rishendra Kumar, A. K. Tiwari and K. S. Bisht

Abstract

The essential oil from the aerial parts of Agrimonia aitchisonii Schonbeck Temesy growing in the Himalayan region is evaluated for its antifungal, antibacterial and antioxidant activity. The oil has shown 100% mycelia growth inhibition against S. sclerotiorum, A. tenuis, C. graminicola and F. oxysporum at a concentration of 2000μg/ml, 2000μg/ml, 3000μg/ml and 3000μg/ml respectively. However R. solani was found less susceptible to the oil of Agrimonia aitchisonii. The IC50 values showed a range from 41.10μg/ml – 85.44μg/ml as compared to standard fungicides with IC50 values 32.8μg/ml – 98.6μg/ml. The spore germination inhibition test has shown the IC50 values as 101.4μg/ml, 314.74μg/ml and 684.7μg/ml for A. tenuis, C. graminicola and F. oxysporum respectively. The ZOI for the test bacterial strains at a concentration of 500μg/ml were Gram negative bacteria Pseudomonas aeruginosa 424 (7mm), Escherichia coli 443 (4mm), Aeromonas hydrophila 646 (8mm) and Gram positive bacteria Bacillus subtilis 441 (4mm) and Staphylococcus aurieus 737 (6mm). The oil has shown 435.7 ± 0.004 mg/ml gallic acid equivalent per 100mg of the oil as total phenolic content and 423 ± 0.003 mg/ml quercetin equivalent per 100 mg of the oil as total flavonoid content. The VEAC values were found to be 0.454 ± 0.003g/L and 0.538 ± 0.001g/L for the essential oil and ascorbic acid respectively.

Keywords: Agrimonia, essential oil, ailments, antioxidant, antibacterial activity, infections.