Abstract

STANDARDISATION OF ONE OF TRADITIONAL ‘DASHMULA’: SHALPARNI (DESMODIUM GANGETICUM LINN. DC. FAMILY: LEGUMINOSAE) THROUGH PHYTOCHEMICAL AND HISTOLOGICAL EVALUATION

Attarde D.L.*, Dr. Pal S.C. and Dr. Bhambar R.S.

Abstract

Desmodium gangeticum (L.) DC. Belonging to Leguminosae commonly known as Salparni one of laghupanch -mool from traditional Ayurvedic ‘dashmool’contains sterols, flavonoids, pterocarpan, lipids, glycolipids and alkaloids. Advances in microscope technology like Scanning Electron Microscope have increased the accuracy and capabilities of microscopy as a mean of botanical identification. The objective of this work to provide set of SEM histological characters that may serve as standard for identification. That helps for establishment of botanical biomarkers based on the major microscopic features observed in D. gangeticum. Secondly physicochemical parameter was evaluated. Phytochemical screening petroleum ether, chloroform, alcoholic and ethyl acetate extracts were performed. HPTLC fingerprinting of DG-AL and DG-ET extract in mobile phase Toluene:Ethyl acetate :Formic acid (6:4:0.3),scanned at 262nm shows 11 and 7 zone with good resolution.. DG-ET extract was marked qualitatively with Genistein isoflavone as standard at Rf 0.49. Stability of DGET in solution and over plate with time was checked shows persistent chromatogram. Stability during chromatography checked with 2D chromatogram shows all zone in diagonal line. This article focused for qualitative fingerprinting of genistein in extracts of shalparni dashmul and histological evaluation for development of quality parameter as standardize aspect.

Keywords: Desmodium gagneticum, HPTLC fingerprinting, SEM images, Dashmula, Genistein.