Abstract

SYNTHESIS OF SILVER NANOPARTICLES USING ADATHODA VASICA AQUEOUS LEAF EXTRACT AND ITS EFFECT ON BACTERIAL ACTIVITY

B. Ramya, S. Seetharaman, V. Indra*, K. Perinbam and S. Geetha

Abstract

The silver nanoparticles (AgNPs) were synthesized using aqueous Adathoda vasica leaf extract as reducing and stabilizing agent are reported and evaluated for antibacterial activity against gram positive and gram negative bacterial species such Salmonella typhi, Bacillus subtilis. The nature of silver nanoparticles was analyzed by UV-visible spectrum, FT-IR, XRD, EDAX and TEM. The AgNPs were with an average size of 45 to 50 nm in size and mostly spherical in shape. The antibacterial activity of synthesized AgNPs was compared with that of aqueous extract of A. vasica by disc diffusion method. The biosynthesized AgNPs showed significantly inhibited bacterial growth against (14mm) Salmonella typhi and Bacillus subtilis (12 mm). This study revealed that aqueous leaf extract of A.vasica showed minimum inhibitory zone than the silver nanoparticles used. Thus AgNPs showed broad spectrum and antibacterial activity at lower concentration (1mM AgNO3 in 100 ml) and may be alternative ecofriendly, nontoxic, conventional therapeutic approach in future.

Keywords: A. vasica, UV – Vis spectrophotometer, FT-IR, XRD, EDAX and TEM.