Abstract

ANTI-TUMOR AND ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PRODUCED BY STREPTOMYCES SPP

Bassam Qasim* and Mohsan H. Risan

Abstract

Actinomycetes produce a wide range of secondary metabolites and more than 70% of the naturally derived antibiotics that are currently in clinical use are derived from soil. Actinomycetes and increasing of the pathogens resistant against antimicrobial agents becomes a health problem, so the current study was aimed to isolation of local Streptomyces spp. that is capable of producing secondary metabolite and investigate their biological antimicrobial and anticancer activities. All isolates were tested for antimicrobial activities. Among all suspected Streptomyces (Bag3) was able to inhibit the growth at least two of tested microorganisms. The MICs potential against Staphylococcus aureus and Escherichia coli were 8 and 16 μl/ml respectively, the extracellular crude show strong inhibitory activity against Gram-positive bacteria and moderate inhibitory activity against both gram-negative bacteria. The antitumor activities were determined by MTT assay, which indicated Bag3 possesses a powerful effect against used cancerous cell lines, especially against (Hep G2) by inhibiting 65.75% (400 μg /ml) of cancer cells after 72 hours of incubation, while against the (SUN16) 42.84% (400 μg /ml)and against the WRL-68 (normal cell line) 13.43% (400 μg/ml) this indicate the effect of extracellular crude extract was achieved with concentration -dependent manner (dose dependent). This means that the isolation bacteria (Bag3) have little effect on normal cells, because we need high concentrations to effect on the normal cells.

Keywords: Anti-tumor, Antimicrobial, Streptomyces.