Abstract

EVALUATION OF THE GROWTH PROMOTING POTENCY OF 6 DIFFERENT SYNTHETIC PEPTIDES USING RAMA-27 CELL-LINE

Noorhan Ali Chelebi*

Abstract

Six versions of chemically synthesized peptides similar to milk peptides that have nutriceutical, therapeutic and cosmetic (anti-aging) properties were prepared by peptide synthesizer in the laboratory and subjected to bioassay method involved rat mammary gland cells (Rama-27) in vitro. Both molecular weights and sequences of these peptides were confirmed using Positive Ion Electroscopy Mass Spectrometry analysis by Pepsyn's standard operating procedure for peptide synthesis. Cell culture techniques with doses ranging from (3.125ng/mL-450μg/mL) of these peptides dissolved in normal phosphate buffer saline (PBS) of pH 7.2 showed, alone, an insignificant effects as a growth promoting factor onto propagation of Rama-27 cells. Various doses of standard natural product (SNP), the extract of cow whey, alone, or mixed with equal volume of the synthesized peptides produced significantly higher growth promoting effects at doses 25-50 μg/mL. These results suggest that synthesized peptides are, alone, inactive and do lack the key co-factors as complementary elements to stimulate cell proliferation in vitro, unless mixed with other elements i.e. fetal calf serum (FCS) and SNP or alkaline buffer to activate or promote their growth activity characteristics. It is concluded that the composition of these peptides does still lack the natural key element to possess a full potency as a growth factor.

Keywords: Growth promoting factors, Rama-27, Synthesized peptides, in vitro.