Abstract

PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DENDROCALAMUS STRICTUS LEAVES EXTRACTS

S. N. Wangawar, G. R. Shendarkar*, D. P. Shelke, A. K. Daswad, J. G. Pohare and A. B. Roge

Abstract

This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Dendrocalamus strictus. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of aqueous and ethanolic extracts of leaves of Dendrocalamus strictus (an ethno medicinal plant) was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using agar cup method. The antibacterial and antifungal activities of extracts (50 & 100μg/ml) of Dendrocalamus strictus were tested against two Gram-positive— Staphylococcus aureus, Bacillus subtilis; two Gram-negative—Escherichia coli, Salmonella typhi human pathogenic bacteria; and four fungal strains—Aspergillus niger, Penicillium chrysogenum Aspergillus flvus, Fusarium moneliforme. Zone of inhibition of extracts were compared with penicillin for antibacterial activity and decrease in growth of fungi was compared with griseofulvin for antifungal activity. The results showed that the remarkable inhibition of the bacterial growth was shown by ethanolic extract at both the concentrations (50 & 100μg/ml). The Phytochemical analyses of the plants were carried out. The microbial activity of the Dendrocalamus strictus was due to the presence of various secondary metabolites. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.

Keywords: Dendrocalamus strictus, Phytochemical screening, Antibacterial, Antifungal activity.