Abstract

PHYTOCHEMICAL SCREENING AND IN-VITRO ANTI-OXIDANT ACTIVITY OF METHANOLIC LEAF EXTRACT OF COLDENIA PROCUMBENS LINN.

Ch. Malathi Suvarna*, B. Santosh, Ch. Sireesha and Ch. Sarada Durga

Abstract

Anti-oxidant plays a major role in the prevention of carcinogenesis. In the present study we tried evaluating the anti oxidant activity of methanolic extract of Coldenia procumbense leave. The phytochemical screening indicated the presence of steroids, flavonoids, Alkaloids, Phenols, Glycosides in the methanolic extract. Antioxidant value of the extract was evaluated by using Hydrogen peroxide radical scavenging assay and reducing power method. In both the methods Ascorbic acid is used as standard and it was observed that Coldenia procumbense showed considerable inhibition (84 - 95%) when compared with that of standard. Reducing power of extract was increased with concentration thus showing that the antioxidant potential is dose dependent. Thus proving that the methanolic extract of Coldenia procumbens leaves is capable of antioxidant activity even though further detailed molecular level study is in need.

Keywords: Coldenia procumbens, Phytochemical Screening, Antioxidant, Hydrogen peroxide, Reducing Power Method.