Abstract

EVALUATION OF ANTI-OXIDANT AND ANTIBACTERIAL ACTIVITY OF POMEGRANATE (PUNICA GRANATUM L.) SEED EXTRACT

*Talpade M. B. and Singh A. S.

Abstract

In the present study Aqueous, Methanol, Acetone and Ethyl acetate extract of seeds of Punica granatum were prepared and evaluated for its antioxidant and antibacterial properties against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Enterobacter aerogenes, Pseudomonas aeruginosa. The results showed the highest extraction efficiencies were for Methanol and Acetone solvents in extraction of seed. The methanol extract has shown highest antibacterial activity compared to other extracts. Among the selected bacterial cultures, the highest antibacterial activity was recorded against Staphylococcus aureus, Escherichia coli. Antioxidative study was evaluated by DPPH assay. DPPH assay showed potential antioxidative activity by the various extracts. It can be concluded Pomegranate seed, which possesses high levels sources of the natural antioxidants with potent antibacterial properties.

Keywords: Pomegranate seed, Antioxidant, antibacterial activity, bacterial pathogens.