Abstract

ANTIMICROBIAL ACTIVITY OF ACTINOMYCETES ISOLATED FROM RHIZOSPHERE SOIL OF INDIAN MEDICINAL PLANTS AGAINST HUMAN SKIN PATHOGENS

*Raut R. A., D. D. Karad and S. W. Kulkarni

Abstract

Actinomycetes also called as “actinobacteria” are primarily distributed in natural ecosystems all over the world. The aim of this study is to search antimicrobial activity of actinomycetes isolated from some Indian medicinal plants rhizosphere soil samples. Total five rhizosphere soil samples of medicinal plants viz; Aloe barbadense, Emblica officinalis, Zingiber officinale, Eucalyptus camaldulensis and Santalum album. These medicinal plants were collected from local area at Barshi.Dist-Solapur, M.S.India. Total thirty-two isolates were obtained and studied by morphologically, culturally and biochemically. All isolates were identified as actinomycetes with the help of MICROIS Software and also few by 16rRNA analysis. All these isolates were belonged to Streptomyces, Streptoverticillium, Nocardia, Micromonospora and Micropolyspora. The antimicrobial activity were tested against standard culture of human skin pathogens such as Candida albicans, Acinetobacter baumannii and Aspergillus niger. All actinomycetes were tested for their antimicrobial activity against all pathogens by agar overlay technique. Streptomyces enissocaesilis (AL3) isolated from Aloevera barbadense was showed antimicrobial activity against Candida albicans, Acinetobacter baumannii and Aspergillus niger. Streptomyces chattanoogensis (SD3) isolated from Santalum album was showed maximum zone of inhibition against Candida albicans.

Keywords: Indian medicinal plants, antimicrobial activity, human skin pathogens.