Abstract

INDIRECT ORGANOGENESIS OF ANDROGRAPHIS ECHIOIDES (L.) NEES

Dr. T. Francis Xavier and D. Sathish Kumar*

Abstract

In present study, suitable protocol for callus induction and regeneration for the valuable medicinal plant species, Andrographis echioides has been developed by micropropagation technique. Leaf and node explants inoculated on MS medium containing the growth regulator (2,4-D 3.0mg/L) + (NAA 2.0mg/L) respectively showed higher callus induction (Leaf explants 90%) and (node explants 95%). The amount of the callus responded for shoot formation grater was leaf callus explant 93% (28.14±4.05) and shoot callus explant 87% (22.30±3.30). When the sub cultured on to the MS medium containing BAP (3.0mg/L) the best shoot formation leaf callus explant 84% (22.07±4.55) and shoot callus explant 89% (29.85±6.93). When the sub cultured on to the MS medium containing KN (5.0mg/L) the elongation shoots were rooted well on MS medium supplemented with IBA (1.5mg/L) and IAA (2.0mg/L). Regenerated plantlet were successfully acclimatized and hardened off in green house condition with better survival rate of 70%.

Keywords: Andrographis echioides, growth hormone, indirect organogenesis.