Abstract

PROTECTIVE ROLE OF MORINGA OLEIFERA LEAF EXTRACT AS A NATURAL ANTIOXIDANT AGAINST TOXIC EFFECT OF PARACETAMOL ON MALE MICE

Samia A. El-Fiky, Nagwa A. Hassan, Hassab El-Nabi S. E., Hasnaa A. Radwan* and Elsayad R. I.

Abstract

Moringa oleifera Lam. is a tree that grows widely in many tropical and subtropical countries. It belongs to Moringaceae family. Moringa oleifera has been reported to possess anti-cancer, anti-inflammatory and antioxidant effect. The present study was conducted to assess the biochemical and cytogenotoxic effects induced by antipyretic drug Paracetamol on both somatic cells and sperms of male mice and determination of the possible protective role of Moringa oleifera as natural antioxidant. Adult male mice of fifteen groups (fifteen animals each) were used in this study: negative control; saline group;. Groups 3-6 received Paracetamol at single dose 500, 7 consecutive doses of 500 for seven days, single dose 2000 and7 consecutive doses of 2000 mg / kg for seven days, group 7 received 200 mg/kg. of Moringa for ten consecutive days, groups 8-11 received Moringa before administration of different doses of paracetamol and 12-15 received moringa after administration of the different doses of paracetaml. Reduced glutathione content in liver tissue, frequency of chromosomal aberrations in bone marrow cells and sperm abnormalities were analyzed. The results showed that the paracetamol treatment caused depletion in reduced glutathione content, significant induction of chromosomal aberrations and sperm abnormalities Oral administration of moringa oleifera leaf extract either before or after paracetamol treatment was effective in improving the content of hepatic reduced glutathione content (GSH); moringa oleifera leaf extract was effective in the reduction of chromosomal aberrations of bone marrow cells and total morphological sperm abnormalities.

Keywords: Moringa oleifera leaf extract, Paracetamol, Reduced Glutathione, Chromosomal aberrations and Sperm abnormality.