Abstract

ISOLATION AND MOLECULAR IDENTIFICATION OF HALOPHILIC ARCHAEA (Part –I) FROM SALINE SOIL

Arpita R. Dave* and Dr. Ajit V. Pandya

Abstract

Prokaryotics taxonomy is consider as a dynamic science. A handful of soil may contain many prokaryotic species. Due to molecular systematic, which led to the splitting of prokaryotes into bacteria and archaea. As molecular systematists continue to work on the phylogeny of prokaryotes. The use of polymerase chain reaction (PCR) has allowed for more rapid sequencing of prokaryote genomes. Archaea are highly diverse with respect to morphology, physiology, reproduction and ecology. As they are best known for growth in anaerobic, hypersaline, pH extremes, and high temperature habitats. Also found in marine arctic temperature and tropical waters. The research work in this is divided in two stages 1) Isolation and characterization of halophilic archaea 2) Phylogenetic identification of the haloarchaeal isolates. Section I is the introduction part Section II describes materials and methods for isolation, morphological and cultural characterization, cultivation and phylogenetic analysis of isolates. In Section III the experimental results showing cultural characteristics, growth requirements, phylogenetic trees and discussion with conclusion is included.

Keywords: Extremophiles, Archaea, Osmolytes, Phylogenetic Analysis.