Abstract

CHARACTERIZATION OF SALMONELLA SPP– PRODUCING EXTENDED-SPECTRUM Β-LACTAMASE (ESBL) ISOLATED FROM CHICKEN SLAUGHTERHOUSES PROCESSING LINE IN NAMAKKAL

*R. Sowmiyadevi, M. Anitha, D. Jegadeeshkumar and B. Madhumathi

Abstract

The aim of the current work was to evaluate the prevalence, antimicrobial susceptibility biofilm formation and ESBL producing Salmonella species isolated from different poultry meat and poultry processing line. The sampling at slaughterhouses was performed for Knife, blender, Chopping board, poultry feed, feces, cage and meat. Totally 32 isolates were identified as Salmonella typhi and Salmonella typhimurium and were tested for susceptibility to 10 antimicrobial agents by the disk diffusion method. The highest probability of antimicrobial resistance occurrence of Salmonella spp was noticed for Gentamyicin and second most was kanamycin and Ciprofloxacin. A more frequent profile of multidrug resistance was noticed for isolates from cutting board, fecal and meat samples. In addition, 54% and 61.5% of biofilm and betalactamase producers were observed respectively. These results reinforce the need of efficient strategy implementation to control and reduce Salmonella spp in chickens at slaughter levels, and the necessity to reduce the use of antimicrobials in the poultry sector.

Keywords: Salmonella spp, Biofilm, Betalactamase, ESBL, poultry processing line.