Abstract

SPECTROPHOTOMETRIC AND RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF LEVOFLOXACIN

Kauser Fatema*, Sadhana Shahi, Tauqeer Shaikh and Nityanand Zadbuke

Abstract

In this study, a simple, sensitive and highly accurate ultraviolet spectrophotometric and RP-HPLC method has been developed and validated for determination of levofloxacin in bulk and pharmaceutical formulations. The method is based on the measurement of the absorbance of levofloxacin solution in 0.1N HCL at 294nm. Beer’s law was obeyed in the concentration range of 2-12μg/ml. The slope, intercept and correlation coefficient were also calculated. For HPLC method validation the analyte was resolved by using a mobile phase [sodium acetate buffer and acetonitrile was selected in a ratio of (60:40) respectively] at a flow rate of 0.8ml/min, with a column at a wavelength of 295nm. The linear dynamic range for levofloxacin was 2μg-8μg/ml.

Keywords: Levofloxacin, Spectrophotometry, HPLC, linearity, validation.