Abstract

ACIDIC CHITINASE PRODUCTION BY NEISSERIA SPECIES

R. S. Mane*, G. S. Patil and B. R. Choradiya

Abstract

The soil sample was collected from the Miraj (MS) fish market. The soil sample was enriched in minimal salt medium containing chitin as sole carbon source. 30 Bacterial species were isolated from enriched soil sample by serial dilution technique and spread plate technique on chitin agar medium. The isolated bacterial species were screened for chitinase production by primary and secondary screening. In the primary screening chitinase production were confirmed by hydrolysis zone and in secondary screening submerged fermentation were used for the chitinase production by using 1% colloidal chitin in nutrient broth. Among the 30 bacterial colonies R4 shown highest zone of hydrolysis and maximum chitinase activity at pH 6.0. Further R4 were characterized and identified as a Neisseria species according to Bergeys manual of Bacteriology.

Keywords: Chitinase, Chitin, Screening, Neisseria species.