Abstract

DESTAINING POTENTIAL OF BACTERIAL LIPASE ENZYME ISOLATED FROM PROVIDENCIA RETTGERI INHABITING THE FRESH WATER FISH MYSTUS BLEEKERI

N. Deepa, R. Usha, A. Anbarasu, K. Anuanandhi, P. Karnan and K. Elumalai*

Abstract

The objectives of present study were to isolate and identify lipaseproducing bacterial strain Providencia rettgeri (MB3) was isolatedfrom fresh water fish Mystus bleekeri. Total of 6 bacterial strains wereobtained in the fish tissues. Screenings of lipase production were testedon tributyrin agar plate. Among the 6 isolates, MB3 was showedmaximum zone of hydrolysis around the well. Partial purification ofcrude enzyme produced from P. rettgeri (MB3) was carried out byammonium sulfate precipitation and dialysis further purified forDEAE-Cellulose column chromatography. The optimization of lipaseenzyme was active at pH 8, temperature 40°C, incubation 48 hrs,carbon source as lactose and best nitrogen source was peptone.According to our knowledge, this study demonstrated the first report on alkaline lipaseproducing to P. rettgeri (MB3) isolated from fresh water fish. The partially purified lipaseenzyme was effectively removed by the blood stains and paves the way for its possibleutilization in textile industry in the near future.

Keywords: Lipase enzyme, Providencia rettgeri, Mystus bleekeri, Optimization, Blood stain.