Abstract

EFFECT OF POLARITY OF SOLVENTS ON CINNAMOMUM ZEYLANICUM BARK EXTRACTION

Vijender Singh*, Sanjeev Kumar Gupta and Anita Dua

Abstract

Cinnamomum zeylanicum bark (CZB) powder was extracted in milli-Q-water, 60% methanol and ethyl acetate with polarity 10.2, 8.2 and4.4 respectively on Tarsons’s spinix orbital shaker at room temperatureto inspect their various potent antioxidant properties. Extractedpolyphenols from CZB in milli-Q-water, 60% methanol and ethylacetate were 39.03 ± 4.59, 84.31 ± 7.33 and 18.85 ± 2.17mg gallic acidequivalent (GAE)/g dry bark weight respectively as major antioxidantcompounds. The flavonoid compounds were 18.35 ± 0.90, 27.12 ±1.50 and, 9.19 ± 1.08mg quercetin equivalent (QE)/g dry bark in milli-Q-water, 60% methanol and ethyl acetate respectively. Extracts wereassayed for DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity and metalinduced lipids peroxidation inhibition for the antioxidants properties. Presence of the extractequivalent to 1μg dry bark extract could protect DNA (deoxyribonucleic acid) againstdamage under H2O2 induced oxidative stress. The results of this study indicate that extract in60% methanol had more polyphenols and exhibited better free radical scavenging, reducingand metal chelating activity, which stand to protect biomolecules like lipids and DNA againstoxidative stress.

Keywords: Polyphenols, flavonoids, Cinnamomum zeylanicum, DNA, oxidative stress.