Abstract

PREPARATION OF SILVER NANO PARTICLES FROM THE BARK OF ALSTONIA SCHOLARIS AND EVALUATION OF ITS ANTIEPILEPTIC ACTIVITY

Narasimhachar Joshi*, Imad Uddin MD, Zeba Ahmedi, Y. Ravali, Veena and V. Bhavani Reddy

Abstract

Devil tree commonly known as alstoniascholaris belonging to family Apocynaceae. Alstonia species are used as medicinal agent for the treatment of various diseases. This study was conducted to develop an ecofriendly, cheap and effective procedure for the green synthesis of silver nanoparticles (AgNPs) form alstonia bark aqueous extract (ASAE) and evaluating their in-vitro anti epileptic activity. Addition of ASAE to AgNO3 (1mM) solution kept on stirring at 60oC resulted in the formation of alstonia bark Aqueous Extract Nano Particles (ASAENP). Synthesized nanoparticles are subjected to characterization by various procedures. UV-Visible spectroscopy showed absorption peak at 440nm which was absent in ASAE and AgNO3. FTIR spectroscopy revealed the absorption peaks of different functional groups involved in the formation of ASAENP. Scanning Electron Microscopy (SEM) revealed average size of ASAENP as 115.95nm. 2 𝜃 peaks at 10.60o, 12.65o, 13.02o, 22.86o and 25.50o were identified by X-ray Diffraction studies (XRD). Stability of ASAENP was determined by zeta potential which was found to be -7.4mV. Successfully characterized ASAENP were evaluated for their in-vitro anti epileptic activities. Phenytoin was used as a standard.

Keywords: Alstonia Scholaris, Silver Nanoparticles, Epilepsy, Pentylenetetrazole.