Abstract

IN VITRO MORPHOGENIC STUDY OF LUFFA CYLINDRICA AND CITRULLUS COLOCYNTHIS

Renu Sarin and Sangeeta Samria

Abstract

Luffa cylindrica Linn. and Citrullus colocynthis Linn. are two important plants of family cucurbitaceae. which have not been much investigated for their in vitro morphogenic studies. In the present investigation shoot iniation was reported after 12-15 days of subculturing of callus cultures on MS medium Supplemented with different growth hormones in both the plants. The shoot initiation in L. cylindrica was observed from callus on MS medium supplemented with combination of auxin and cytokinin. The maximum number of shoot production (70.8%) was observed when media was supplemented with Kn + BA (70.8%), and length of shoot was approximately 2.5 cm. Whereas the shoot initiation in C. colocynthis was observed from callus on MS medium supplemented with cytokinin combination. Shooting was reported on BAP+Kn. However, the maximum number of shoot production (75.6%) was observed when media was supplemented with BAP+ Kn, ( 75.6%) length of shoot was approximately 2.6 cm.

Keywords: Luffa cylindrica, Citrullus colocynthis, Multiple shoots, Callus cultures, Growth hormones.