Abstract

ANTI-CONVULSANT ACTIVITY OF SEEDS OF GOSSYPIUM HERBACEUM LINN

Shetti P. P.*, S. S. Jalalpure, Mamatha M. K. and Suma U. S.

Abstract

To investigate the phytochemicl constituents,Anticonvulsant activity and toxicity studies on the seed extract of Gossypium herbaceum Linn. Identification of major chemical constituents such as glycosides, flavonoides and tannins in methanolic and aqueous extracts of seeds of Gossipier herbaceum linn was carried out to confirm the presence of those in the extract. Extract contains Glycosides, carbohydrates, flavonoides, oils & fats and tannins. Particularly flavonoides and phenol compounds as a major chemical constituent. Therefore an attempt was made to separate and isolate the flavonoids from the methanolic extract of seeds of Gossypium Herbaceous. The authenticated seeds of Gossypium herbaceum linn were air dried the uniform powder of seeds of Gossypium herbaceum were subjected to physicochemical characterization parameters and quality control tests of various parameters which are prescribed in the pharmacopoeia literature. The methanolic extract of seeds of Gossypium herbaceum linn was used for separation and isolation of flavonoids by preparative thin layer chromatography. PTZ induced the median lethal dose (LD50) of Gossypium herbaceum was 3000 mg/kg oral administration, respectively. Gossypium herbaceum methanol and aqueous extract produced 83% and 68% protection against convulsion respectively, compared with 100% protection with Diazepam.

Keywords: Gossypium herbaceum Linn, Pentylenetetrazole, flumazenil, GABAAbenzodiazepine, Diazepam, Epilepsy.