Abstract

PHYTOCHEMICAL INVESTIGATION AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF N-HEXANE LEAF EXTRACT OF CLERODENDRUM SERRATUM LINN AGAINST PATHOGENIC BACTERIAL STRAINS

Malik Tafazul Rashid*, A. S. Yadav and Shabir A. Lone

Abstract

Clerodendrum serratum Linn.is widely distributed plant in India and its medicinal use has been mentioned in traditional Indian medicine systems. In the present study phtochemical investigatin and evaluation os antibacterial activity usinf disc diffusion method of n-Hexane leaf extract Clerodendrum serratum Linn was carried out against pathogenic bacteril strains viz., staphylococcus hominis ATCC27844, Pseudomonas putida ATCC2021, Proteus vulgaris ATCC13315, Bacillus subtilis ATCC2063 and Escherichia coli ATCC2065. Among the various concentrations used, 100mg/ml of n-Hexane leaf extract was found to be more effective against all tested microorganisms. The highest activity was recorded aganist Bacillus subtilis ATCC2063 at a concentration of 100mg/ml with zone of inhibition 20±0.25mm and the lowest activity was recorded against Pseudomonas putida ATCC2021 with zone of inhibition17.1±0.40mm. At a concentration of 25mg/ml, the maximum inhibitory activity was recorded against Escherichia coli ATCC2065 with zone of inhibition 3.62±0.51mm and the minimum inhibitory activity was recorded against Pseudomonas putida ATCC2021 with zone of inhibition 2.5±0.30mm. Therefore, it was found that the inhibitory activity of n-Hexane leaf extract of Clerodendrum serratum Linn. was concentration dependent.

Keywords: Clerodendrum Serratum Linn, Staphylococcus Hominis, Pseudomonas Putida, Proteus Vulgaris, Bacillus Subtilis and Escherichia Coli.