Abstract

EFFECT OF METHYL PARATHION (AN ORGANOPHOSPHATE) ON ELECTROPHORETIC PATTERNS OF PROTEINS IN GILL AND MUSCLE OF FRESH WATER CAT FISH HETEROPNEUSTES FOSSILIS (BLOCH)

Bheem Rao T., Thirupathi K. and Venkaiah Yanamala*

Abstract

The present study was carried out to determine the effect of Methyl parathion (an organophosphate) on electrophoretic patterns of proteins in the gill and muscle of freshwater cat fish H.fossilis (Bloch). The fish were exposed to 2% sub lethal concentration of pesticide Methyl parathion at different time intervals i.e. 24 h; 48 h; 72 h and 96 h for a period of 10 days. The changes in the tissue proteins of vital organs such as gill and muscle were examined on 7.5% of SDS –PAGE. The protein patterns indicated that the muscle has higher number of protein bands compared to gill and control. The number of protein bands observed in gill of test fishes were found to be lesser than the muscle control. The protein banding patterns were identified by standard marker protein and Rm values were calculated accordingly. The results of the present study of electrophoretogram of both gill and muscle showed homology in protein bands with minor variations.

Keywords: Protein patterns, Methyl parathion, SDS –PAGE, H. fossilis.