Abstract

IN SILICO DRUG DESIGN AND EXTRACTION OF PIPERINE AN INHIBITOR FOR FERNESYLTRANSFERASE IN CRYPTOCOCCUS NEOFORMANS

V. Mohanraj, B. Aravindan, C. Jayaprakash and M.Thenmozhi

Abstract

Piper nigrum (black pepper) extract contains several important alkaloids. These alkaloids are important in medicinal biology. In this present work we have attempted to extract an important component from black pepper by solvent extraction procedure. Extracted product was isolated, purified and identified by TLC,UV-VIS spectroscopy, FT-IR Spectroscopy and 1H-NMR, 13C-NMR spectroscopy. Finally, the isolated compound of piperine activity was checked and proved through the insilico analysis using HEX software. The synthesized compound piperine was used to stop the function of the protein farnesyltransferase available in the disease causing organism Cryptococcus neoformans and also compared with the existing drugs.

Keywords: 1. Piper nigrum 2. Dichloromethane 3. Molecular docking 4. Cryptococcus neoformans.