Abstract

MODULATING EFFECTS OF CURCUMA LONGA (TURMERIC) EXTRACT AGAINST ADRIAMYCIN INDUCED GENOTOXICITY IN SWISS ALBINO MICE

J. Karuna Kumari* and K. Rudrama Devi

Abstract

In the present investigation, studies were carried out to observe the modulating effects of crude Curcuma longa rhizome extract (CRE) against Adriamycin (ADR) induced genotoxicity in germ cells of mice using Sperm Morphology Assay. Two experiments were conducted in germ cells of Swiss albino male mice. Three different doses 150, 250 and 350mg/kg body weight of the Curcuma longa extract and 16mg/kg body weight Adriamycin were taken for study based on therapeutic concentration., in first test the antigenotoxic effects of Curcuma longa were studied and in the second test one group of animals were treated with Adriamycin alone and other groups of animals were primed with Curcuma longa extract prior to adriamycin treatment. Control animals were fed only with physiological saline. All the exposed and control Animals were sacrificed on last day of fifth week and cauda epididymis was dissected out, smear was made and stained with Eosin, slides were prepared and screened for the presence sperm abnormalities. The results were found to be insignificant, where Curcuma longa extract proved to be antigenotoxic in treated animals A significant increase in the percentage of abnormal sperms were noted when treated with Adriamycin alone and the cells showed inhibition in the percentage of abnormal sperms when animals were primed with Curcuma longa extract prior to adriamycin treatment. The present study reveals that the Curcuma longa extract was found to be antimutagenic. Hence Cucuma longa supplementation is safer in chemotherapeutic strategy.

Keywords: Curcuma longa, genotoxicity, Adriamycin, germcells, Swiss albino mice.