Abstract

A STUDY ON PROTEASE AND LIPASE PRODUCING BACTERIA FROM OIL CONTAMINATION SOIL AND SCREENING OF PYOCYANIN PIGMENT

Yazhini Jagadeesn* and Prabavathi S.

Abstract

Oil spilled soil samples were collected from different places of Tamil Nadu such as Salem, Chennai, Kanchipuram, Vizhupuram and Vellore. The collected oil contaminated soil samples were used for isolation of protease and lipase producing Pseudomonas strains. Qualitative analysis of protease enzyme proteolytic zone was recorded in the oil spilled soil sample collected from Salem which recorded 3.0 cm followed by Villupuram, Vellore, Kanchipuram and Chennai. Qualitative analysis of lipase enzyme Lipolytic zone was recorded in the oil spilled soil sample collected from Salem followed by Vellore, Villupuram, Chennai and Kanchipuram. Quantitative assay of protease enzyme from five pseudomonas species were studied. Among five different places and five pseudomonas species, the maximum proteolytic activity was found maximum in pseudomonas speciesisolated from oil spilled soil sample collected from Salem followed by Villupuram, Vellore, Kanchipuram and Chennai. Quantitative assay of lipase enzyme from five pseudomonas species were studied. Among five different places and five pseudomonas species, the maximum lipolytic activity was found maximum in pseudomonas species isolated from oil spilled soil sample collected from Salem followed by Vellore, Villupuram, Chennai and Kanchipuram. Among five different places and five pseudomonas species were studied for pyocyanin pigment production. The maximum pyocyanin pigment production was found maximum in pseudomonas species isolated from oil spilled soil sample collected from Salem followed by Vellore, Kanchipuram, Villupuram and Chennai. Seed germination test: In this study, soil sample contaminated with oil and the same soil sample treated with the supernatant extracted from the isolated culture of Pseudomonas species. Were used for the seed germination test. Two different Individual soil sample were used, one sample was oil spill contaminated soil and the other was treated oil spilled soil. Both of the were used for seed germination and growth studies. The clean surface sterilized seeds (25 number of green gram seed) were inoculated on oil spill contaminated soil and treated oil spilled soil. The sterile seeds were maculated into different petriplate, the maximum response of seed germination 85% and development of shoot length 5.3 m per plant were found in soil treated with enzyme solution followed by untreated 50. Thus to conclude that treated oil spill soil show more enzyme activity and excellent seed germination quality.

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