Abstract

SYNERGISTIC EFFECT OF PARTIALLY PURIFIED BACTERIOCIN FROM CRONOBACTER SAKAZAKII AND ANTIBIOTICS AGAINST STAPHYLOCOCCUS AUREUS

Nedhaal S. Zbar, Mahaba R. Al-Roubaiee*, Hameed M. Jasim

Abstract

The antibacterial activity of partially purified bacteriocin produced by Cronobacter sakazakii was studied by well diffusion method. Results showed that bacteriocin produced by C. sakazakii have the antibacterial activity according to the inhibition zones against E. coli, S. aureus, K. penumoniae, S.dysenteriae, P. vulgaris and S. marcescens. Antibiotic susceptibility of S. aureus was examined against different antibiotics. The minimum inhibitory concentration of bacteriocin, amikacin and tetracycline against S.aureus was determined, then synergistic effect of bacteriocin and antibiotics against S.aureus was analyzed. Result showed that the synergistic effect of bacteriocin with both amikacin and tetracycline in different ratios. The highest synergistic effect was obtained when bacteriocin was mixed with both amikacin and tetracycline in a ratio of 3:1, where the inhibition zones were increased to 29.4 mm and 27.3 mm respectively.

Keywords: Cronobacter sakazakii, bacteriocin, synergistic effect.