Abstract

BIODEGRADATION OF POLY AROMATIC HYDROCARBONS USING NITRIFIFYING PSEUDOMONAS BACTERIA AND ITS MOLECULAR CHARACTERIZATION

S. Priyadharshini and Dr. J. Thirumagal*

Abstract

Biotechnology has applications in four major industrial areas, including health care (medical), crop production and agriculture, nonfood (industrial) uses of crops and other products (e.g. biodegradable plastics, Vegetable oil, biofuels) and environmental uses.To attain the task microorganisms were isolated from the deliming water and air. The efficiency was assured by estimating the level of nitrogen content. It was found that all organisms were capable of performing the denitrifying reaction. The obtained isolates were inoculated in synthetic wastewater and their growth characteristics were studied by varying the pH between 4 to 10. The growth of organisms was maximum in between pH 7 to 9.The isolates were inoculated in deliming water (real life wastewater). Two efficient isolates were selected DN3 and DN5. The isolate DN5 was screened for the utilization of PAHs. The molecular identification of the isolate DN5 was identified based on 16S rDNA sequencing and the sequenced DNA was read in the Genbank databases (BLAST), compared with the other sequences bacterial class and confirmed as Pseudomonas sp.The strain DN5 showed considerable growth with on 1 mM concentration Naphthalene, Phenanthrene, Anthracene, Fluorene, as the sole carbon. Pseudomonas sp. DN5 showed greater degradation capability nearly around 100% against the PAHs tested.

Keywords: Pseudomonas, Denitrification, PAHs, BLAST.