Abstract

BIOREMEDIATION OF POLY AROMATIC HYDROCARBONS BY SELCECTED STAINS OF BACTERIAS

R. Padmini and Dr. J. Thirumagal*

Abstract

Bioremediation is the Biochemical dissolution of materials by bacteria or other biological means. Although often conflated, biodegradable is distinct in meaning from compostable. The ultimate task of the work is to develop a methodology to reduce nitrogen discharge from tannery effluent by microbial mediated nitrification and denitrification. To attain the task microorganisms were isolated from the deliming water and air. The efficiency was assured by estimating the level of nitrogen content. It was found that all organisms were capable of performing the denitrifying reaction. The obtained isolates were inoculated in synthetic wastewater and their growth characteristics were studied by varying the pH between 4 to 10. The growth of organisms was maximum in between pH 7 to 9. The isolates were inoculated in deliming water (real life wastewater). Two efficient isolates were selected DN3 and DN5. The level of nitrogen was estimated in the deliming water by kjeldahl method and noted as 280mg/l. After inoculating the two isolates and 4 days incubation again nitrogen content was checked. The level of nitrogen was reduced for DN3 by 55mg/l and for DN5 by 98mg/l. The isolate DN5 was screened for the utilization of PAHs.

Keywords: Bioremediation, PAHs, Pseudomonas Bacterial Strains, Denitrification.