IN VIVO AND IN VITRO ANTITUMOR ACTIVITY OF A NOVEL PH ACTIVATED POLYMERIC DRUG DELIVERY SYSTEM FOR DOXORUBICIN
Jyoshna Rani Dash*, Bandana Behera, Debasish Pradhan
Abstract
Introduction: Conventional chemotherapy agent together with
Doxorubicin (dox) is of limited clinical use because of its inherently
low selectivity, that could lead to systemic toxicity in regular healthy
tissue. Methods: A pH stimuli-sensitive conjugate based on
polyethylene glycol (PEG) with covalently attachment doxorubicin
through hydrazone bond (peg-hyd-dox) changed into organized for
tumor focused on delivery gadget. while peg-dox conjugates thru amid
bond (peg-ami-dox) turned into synthesized as control. Results: The
artificial conjugates were confirmed by using proton nuclear magnetic
resonance (nmr) spectroscopy, the release profile of dox from peg-hyd dox turned into acidchargeable
for the hydrazone linkage between dox and peg, brought about one-of-a-kind
intracellular uptake direction; intracellular accumulation of peg-hyd-dox became higher than
peg-ami-dox because of its ph-brought about profile, and thereby more cytotoxicity towards
mcf-7, mda-mb-231 (breast most cancers models) and hepg2 (hepatocellular carcinoma
model) mobile lines. following the in vitro outcomes, we xenografted mda-mb-231 mobile
onto scid mice, peg-hyd-dox confirmed stronger antitumor efficacy than loose dox and
became tumor-targeting. Conclusions: effects from those in vivo experiments were regular
with our in vitro effects; cautioned this ph-precipitated peg-hyd-dox conjugate may want to
target dox to tumor tissues and launch unfastened tablets by means of acidic tumor
surroundings, which would be potent in antitumor drug delivery.
Keywords: .
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