Abstract

BACILLUS PUMILUS ISOLATED FROM HEALTHY HONEY BEES AND ITS EFFECTS ON SOME BIOLOGICALASPECTS IN MICE

Abdulaziz A.Y. Abbas* and Abdelmalek M. Amran

Abstract

Several bacterial Strains belonged to Bacillus pumilus have been isolated and identified from several natural sources. Some these the strains are widely applied in industrial microbiology such as probiotics. The aim of the present work was to evaluate the biological activity of B. pumilus isolated from the digestive tract of healthy honey bees as probiotics. The isolation and purification were done using nutrient agar then the primary and secondary identification were performed by HiBacillus TM Identification Kit and 16S rRNA analysis. Procedures and experiments were performed using 60 adult healthy male mice Mus musculus, which were grouped into three groups, the first group was control while the second and third were treated with sterile normal saline solution and B. pumilus suspension of B. pumilus respectively. The results showed that the digestive tracts of honey bees that used in this work contained B. pumilus strains. The data obtained from the animal testing reported that the B. pumilus strain used in this study have a positive impact on the tested biological parameters. The study concluded that the selected B. pumilus strain has a role in a role in improving the health status of mice.

Keywords: Bacillus pumilus, Honey bees, probiotic, mice, biological parameters.