Abstract

BIOSYNTHESIS OF SILVER NANOPARTICLES USING LEAF EXTRACT OF ARAUCARIA HETEROPHYLLA AND ITS ACTIVITY AGAINST BREAST CANCER CELL LINE

Hezinglila Grace* and Albin T. Fleming

Abstract

Background: Biological method of synthesis of nanoparticles using plant extract has proven to provide eco-friendly and satisfactory protocol without the utilization of toxic and costly material. This study investigates an efficient way of synthesizing an AgNPs using Araucaria heterophylla leaf extract and is valued for its medicinal property. Objective: Green synthesis of silver nanoparticle from Araucaria heterophylla leaf extract and its in vitro anticancer activity against breast cancer cell line. Method: Biosynthesized AgNPs were characterized using UV-Vis absorption spectroscopy, SEM, EDX, XRD and cytotoxicity of AgNPs was tested in human breast cancer cell line (MDA-MB- 231). Results: UV-Vis absorption spectrum of silver nanoparticles showed the appearance of absorption band at 428nm. SEM image indicated spherical shape of silver nanoparticles with particle size in the range 120- 200 nm. The EDX spectrum showed the presence of silver along with a few other elements like Na, Al, K, Cl and O. The crystalline nature of the silver nanoparticles was examined by XRD analysis for nano-crystals. Green synthesized AgNPs by Araucaria heterophylla leaf extract showed cytotoxicity to human breast cancer cell line (MDA-MB- 231).Conclusion: The present study reveals that the synthesized AgNPs from aqueous extract of Araucaria heterophylla provide non-toxic, eco- friendly and cost effective reducing and capping agent for bio reduction of AgNO3.

Keywords: Araucaria heterophylla, AgNPs, SEM-EDX, UV-Vis absorption spectroscopy, XRD, cytotoxicity analysis.