Abstract

ANTIPROLIFERATIVE, ANTIOXIDANT, AND ANTIBACTERIAL ACTIVITIES OF CRUDE PLANT EXTRACTS OF ASPHODELINE LUTEA L. AND PEGANUM HARMALA L.

Omar M. Atrooz*, Joanna Wietrzyk, Beata Filip-Psurska, Ibrahim Al-Rawashdeh, Manal Soub and Mohammad H. Abukhalil

Abstract

In this study, we evaluated the biological activity of crude plant extracts of Asphodeline lutea and Peganum harmala. The in vitro antiproliferative activity, the effects on cell cycle phases, and the antioxidant and antibacterial activities of the crude extracts were investigated. The extracts revealed antiproliferative activity against three human cancer cell lines: MDA-MB-231 (breast), Hs-294T (melanoma), MV-4-11 (leukemia),and one non-tumorigenic human mammary gland epithelial cell line, MCF-10A. The crude extract of Asphodeline lutea appeared to be more active against all cell lines tested, while P. harmala extract revealed activity against only the MV-4-11human leukemia cell line. Both extracts differed in their activity toward cell cycle progression. After 72hours of treatment with A. lutea extract, we observed a shift of MV-4-11 cell percentage to the S-phase. On the other hand P. harmala tended to stop the cell cycle in G0/G1 phase. Nevertheless, the changes in cell cycle were not statistically significant when compared with the control group. Moreover, both crude plant extracts exhibited antioxidant activity. Antioxidant capacities of the extracts were expressed in terms of IC50 value of the extracts. Also, the minimum inhibitory concentration (MIC) studies indicated that the MIC of the crude plant extract of P. harmala was found to be 500 μg/ml for all selected bacterial species, while for the crude plant extract of A. lutea, it was found to be 1500 μg/ml for M. luteus, S. aureus, and B. subtilis, and 2000 μg/ml for E. Coli.

Keywords: Plant extracts, Antiproliferative activity, Antioxidant, Antibacterial activity.