Abstract

DEVELOPMENT AND VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF ALBENDAZOLE IN TABLET DOSAGE FORM.

Sandhya Bhimrao Lahane, Dr.U.A.Deokate

Abstract

Albendazole (ABZ) is an oral broad-spectrum anthelmintic, antiparasitic agent generally prescribed for the treatment of tissue infections caused by a variety of nematodes,Threadworm , Hookworm and Tapeworm . Several techniques such as HPLC, HPLC with fluorescence detention, LC-MS, capillary electrophoresis, spectrophotometric, titrimetric and flow injection analysis for the estimation of albendazole alone and with its major metabolites had been reported. Objective: To developed simple, reliable, rapid, precise, specific and reproducible UV spectroscopic method for routine analysis of Albendazole in tablet Form. Method: Aliquots of stock solution were further diluted with DMF to get working solution of 5,10, 15,20,25μsg/ml and the working standards were scanned between 200 - 400 nm which shows the maximum absorbance at 295 nm. Result: The drug obeyed the Beer’s law and showed good correlation. It showed absorption maxima at 298 nm in Dimethyl Formamide (DMF). The linearity was observed between 5-25 μg/ml. The results of analysis were validated by recovery studies. The recovery was found to be 99.43-101.55%. Conclusion: The method was found to be simple, reliable, rapid, precise, specific and reproducible and can be applied for routine analysis of albendazole in different dosage form and dissolution studies.

Keywords: Albendazole, Threadworm, hookworm, spectrophotometry, Beer’s law, recovery study.