Abstract

PHYTOCHEMICAL SCREENING BY UV-VIS, FT-IR AND GC-MS SPECTROSCOPIC ANALYSIS OF LEAF AND CALLUS EXTRACTS OF MEDICINAL PLANTS SIDA SCHIMPERIANA Hochst. ex A. Rich

Prabhu V. and Ramar K.*

Abstract

In the present study an attempt has been made to establish UV-VIS, FT-IR, GC-MS profile and identify the functional components of Sida schimperiana. UV-VIS, FTIR, and GC-MS method was performed on a Thermo Scientific Spectrophotometer system which was used to detect the characteristic peak values and their functional groups.The fresh ethanolic extracts of S. schimperiana Leaves and callus. UV – VIS, FTIR and GC-MS the ethanol extract was examined under visible and UV light for the proximate analysis. The UV‐VIS profile of S. schimperiana Leaves and callus ethanol extract showed the peaks at 643 nm, 647 nm wheels as same callus 425 nm with the absorption of 1000.20, 1000.17 and 521.43 respectively. The FTIR spectrum analysis results proved the presence of Aliphatic compounds, Amine and (amine II bands), Amides and (amine III bands), Benzene ring, Ester and lactones, Sulfides which shows major peaks at 2981.18,1641.10,1404.89, 1329.38, 1272.80, and respectively 703.70. The results of callus FTIR analysis confirmed the presence of, Aliphatic compounds, isocyanates, oximes, Primary amines, Sulfonyl chlorids, Silanes,sulfonic acids, Organo phosphorus compound, Benzenes which shows major peaks at 2986.56, 2085.12, 1640.87, 1406.40, 1394.96, 1259.78, 1066.95, 12045.99 and respectively 878.09. GC-MS analysis of ethanol extracts of S. schimperiana callus analysis the presence of 13 phytochemical compounds. The presence of various functional groups and phytocompounds in S. schimperiana callus confirm that it act as a most important source of drugs against various ailments. The results of the present study produced the UV –VIS, FTIR and GC-MS spectrum profile for the medicinally important plant Sida schimperiana.

Keywords: Sida schimperiana UV- VIS, FTIR, and GC-MS Functional groups.