Abstract

IN VITRO CALLUS INDUCTION AND HISTOCHEMICAL LOCALIZATION STUDIES IN HIBISCUS MICRANTHUS L.

Sharmila Banu M.* and Ramar K.

Abstract

Hibiscus micranthus (L.) is an important medicinal plant which belongs to the family Malvaceae. It is widely used as folk medicine and treating for many diseases. Leaf explants were cultured on MS medium with different concentrations of NAA AND 2, 4-D alone in combination with BAP and KIN for callus induction. Callus initiation was observed in all concentration with huge amount. Highest percentages of callus (87.3%) induction were observed in the combination of BAP (2.0mg/l) NAA (1.0mg/l) 2, 4-D (0.5mg/l) for explants, node respectively. The experimental result of calli was shown as green friable callus, green yellow friable callus and green compact nodular callus of the and nodal segment were cultured on Murashige and Skoog (MS)medium supplemented with BAP (6-benzyl amino purine) NAA naphthalene acetic acid and IAA Idol acetic acid 2,4-Dichlorophenoxyacetic acid (2,4-D). The protocol might be useful for the production of disease free and healthy plant materials. Histochemical localization of H. micranthus. The thin even section were treated with histochemical stains to used by localize substance of carboxylate polysaccharide and lignin indicated giving in specific colors formed the callus. H. micranthus is a medicinal plant leaf explant culture good amount results from callus for the histochemical.

Keywords: Hibiscus micranthus In vitro callus induction, plat growth regulators Histochemical studies.