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Abstract

DEVELOPMENT OF VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ABACAVIR, LAMIVUDINE AND ZIDOVUDINE IN COMBINED TABLET DOSAGE FORM

Vaishali P. Nagulwar, Kishor P. Bhusari

Abstract

Purpose: To develop an accurate, simple and reproducible RP-HPLC method for the simultaneous estimation of abacavir, lamivudine and zidovudine in bulk drug and in combined tablet dosage form. Method: The stock solutions were prepared in acetonitrile and methanol (3:2) followed by the further required dilutions with distilled water. The mobile phase containing methanol: buffer (pH 3.0): acetonitrile: tetrahydrofuran (35:60:5:0.4 v/v/v/v) was used at 271nm at a flow rate of 0.6 mL/min. HPLC model Chemito LC 6600 equipped with Knauer HPLC pump K-501 and Chemito UV-visible detector connected to Chemito’s Chemitochrome C2000 data module, Eurosphere 100-5 C18 (250 mm x 4.6 mm) with precolumn were used in present study. Validation parameters like accuracy, precision, linearity, specificity and ruggedness were performed to ascertain the correctness of the proposed method. Result: The proposed method has estimated abacavir 99.12% ± 0.64, lamivudine 99.55% ± 0.6 and zidovudine 99.76% ± 0.4 in standard mixture and 99.26% ± 0.49, 99.67% ± 0.51 and 99.68% ± 0.58 respectively in the marketed tablets. Conclusion: The present study gives an excellent method for the determination of all the three drugs in combined dosage formulation.

Keywords: ABA, LAM, ZID, RP- HPLC.


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