Abstract

QUANTITATIVE DETERMINATION OF DRUGS AND PHARMACEUTICALS USING CHLORAMINE-T AND METHYL ORANGE AS DYE

B. Vishnuvardhan Reddy and G. Venkateshwarlu*

Abstract

New sensitive and rapid spectrophotometric methods for the determination of five drugs viz., Fosinopril (FOS), Mebeverine Hydrochloride (MEB), Phenylephrine Hydrochloride (PHE), Terazosin Hydrochloride (TER) and Voriconazole (VOR) in pure and pharmaceutical formulations. The proposed methods involve the oxidation of drugs by addition of excess amount of Chloramine-T in acidic medium and subsequent determination of unreacted Chloramine-T using a fixed amount of methyl orange as a dye which absorbance at 510 nm. Beer’s law is obeyed in the concentration of 24-168, 5-35, 12-84, 2-14 and 20-140 μg mL-1 for FOS, MEB, PHE, TER and VOR respectively. Different variables affecting the reaction were studied and optimized. The limits of detection and quantification were also reported for this method.

Keywords: Quantification, Chloramine-T, Methyl Orange, Formulations and Spectrophotometric Determination.